Some USB storage devices do not support Device Identification Inquiry requests and use the same value as the Serial Number Inquiry, or even the same serial descriptor. Multiple LUNs using such devices might not be able to access the bootbank partition of the ESXi host and default to the /tmp directory instead. As a result, ESXi host updates fail.
A unique number assigned to a patent reexamination request when it is filed, having a 2-digit series code (90 for ex parte reexamination requests; 95 for inter partes reexamination requests; 96 for supplemental examination requests), and a 6-digit serial number.
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Unique number assigned to a trademark when it's registered, used to look up and track the registration in our systems and required when filing forms to maintain the registration. The registration number is different from the application serial number.
A six digit number assigned to a patent application when it is filed. A serial number is usually used together with a two digit series code to distinguish each serial set of 1 million applications filed.
(A) Titration curves of non-silencing and LaminA/C shRNA viruses. A673 cells were transduced with serial dilutions of generated lentivirus and GFP expression was analyzed after 72 h by flow cytometry. Functional titers were calculated based on non-shaded dilutions. (B) Titration curves of purchased non-silencing shRNA particles, generated as in (A). (C) Overview of determined functional virus titers [TU / ml] and deduced values. (D) Transduced cell fractions generated with a calculated MOI of 0.3. Left panel: A673 cells were transduced with indicated lentiviruses and GFP expression was analyzed after 72 h. Right panel: Representative flow cytometry plot; shaded graph: transduced cell population; open graph: non-treated control cells. (E) Time-course of puromycin selection. A673 cells transduced with purchased non-silencing shRNA particles in (D) were exposed to puromycin 72 h after transduction. Left panel: GFP-positive cell fractions were determined at time points indicated. Right panel: Representative flow cytometry plot corresponding to that in (D) after 72 h puromycin selection. (F) Western blot of LaminA/C protein expression. A673 cells were transduced as indicated and selected with 1 μg / ml puromycin after 72 h for additional 72 h. Arrows indicate 74 and 65 kDa double-band of LaminA/C. Actin was loading control. All graphs of this figure represent mean SD of three independent experiments.
Cells were seeded into 24-well plates at above densities. One day later, one well was counted to determine cell number. Other wells were transduced with serial dilutions of lentivirus as outlined above in a total volume of 200 μl. After 24 h, 800 μl of standard growth medium was added. Transduced cell fractions were determined after 72 h. The functional titer expressed in transducing units per ml [TU / ml] was calculated as: (GFP-positive cell fraction x total cell number at transduction x dilution factor) transduction volume. Calculations were performed for four dilution factors of the serial dilution and three independent experiments to determine mean functional titers. Functional titers of purchased non-silencing shRNA lentivirus served to calculate the relative transduction efficiency of A673 and HEK293 target cell lines as: functional titer [TU / ml] virus-batch reference titer [TU / ml]. The anticipated titer of the Decode Pooled Lentiviral shRNA Library was deduced as: relative transduction efficiency x reference titer [TU / ml].
You can monitor the serial activity at a GPIO with a connected LED. This function is useful for debugging purposes and also to see data is coming in during normal operation. Usage: Enter the command sensor53 lx to activate this function (Lowercase L for LED). x is the number of the GPIO where the LED is connected. For example you can use sensor53 l2 for the onboard LED on a Wemos D1-mini or sensor53 l13 on a Sonoff Basic. sensor53 l255 turns the function off. This is the default value. With sensor53 mx you can choose which serial meter (x) will be monitored. Set sensor53 m0 will monitor all serial meters. This is the default value. To start the monitoring at boot-time, simply add the necessary entries in the boot-section (>B) of the script:
You can dump to your PC the raw data coming in if you use the module's hardware serial ports (1 and 3) as GPIOs of the script, using Serial to TCP Bridge. Compile your firmware with USE_TCP_BRIDGE, disable the script and configure in module parameters TCP Tx and TCP Rx. After module reboot, start the server with command TCPStart 8888. Connect to this port from your PC to see or dump the data, in Linux it's as easy as cat rawdump.txt. To revert to SML you need to set back both GPIO ports to None, enable the script and restart.
Also, the source code has to be patched from 8N1 to 7E1 mode for the hardware serial in file src/TasmotaSerial.cpp, please see the patch further down below. Since Tasmota 9.5.0 the serial mode can be changed in the console by typing SerialConfig 7E1 without having to patch TasmotaSerial.
The meter's manufacturer's datasheet neatly explains the serial message format used, so you can easily adapt the code below to your EasyMeter Q1D, e.g. if you have a two-way counter variant like the EasyMeter Q1DA1026.
For Tasmota versions that are built with a TasmotaSerial.cpp of version 3.5.0 (and probably all higher versions, too), no modification of the TasmotaSerial.cpp source code (as suggested in other entries of this documentation) is necessary to set the serial parameters to 7E1: By configuring the meter type as OBIS ("o") in line 5 of the above code, you implicitly tell Tasmota to set the serial parameters to 7E1 (probably the same applies to all other meters in this documentation where a modification of TasmotaSerial.cpp has previously been recommended).
This heat meter needs a wakeup sequence with 2400 Baud 8N1, wheras communication is done by 2400 Baud 8E1. The script will therefore only rund starting with Tasmota 12.2 where switching parity is implemented. For compiling, add the following to your user_config_override.h to increase serial communication buffer size and enable MQTT and Web publishing: 2ff7e9595c
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